Coding

Part:BBa_K3256442:Design

Designed by: Ru Ying Hung   Group: iGEM19_NCTU_Formosa   (2019-08-18)


YafQ*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 129
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Be sure that there is no any normal restriction site in the sequence


Source

https://www.uniprot.org/uniprot/Q47149

References

Prysak, M. H., et al. (2009). "Bacterial toxin YafQ is an endoribonuclease that associates with the ribosome and blocks translation elongation through sequence‐specific and frame‐dependent mRNA cleavage." Molecular Microbiology 71(5): 1071-1087.